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Objective To observe the visual acuity change in patients with different patterns of optical coherence tomography (OCT) of diabetic macular edema (DME) after intravitreal ranibizumab injection and/or laser photocoagulation.Methods A retrospective observational case series.Seventy patients (99 eyes) with DME were enrolled.Best-corrected visual acuity (BCVA) was evaluated using the international vision test chart,and then convert the result to the logarithm of the minimum angle of resolution (logMAR).According to the morphological characteristics of OCT,the DME was divided into 3 patterns,including diffuse macular edema (DRT),cystoid macular edema (CME) and serous neuroepithelial layer detachment.The average follow-up was (80.43 ± 74.89) days.The patients were divided into 3 groups according to the different treatments,including intravitreal ranibizumab injection group (group A,21 patients,25 eyes),intravitreal ranibizumab injection and laser photocoagulation group (group B,23 patients,26 eyes),laser photocoagulation group (group C,26 patients,48 eyes).The changes of absolute BCVA (ABCVA) and improved visual acuity were compared between different treatment groups and different OCT patterns.ABCVA =logMAR BCVA before treatment-logMAR BCVA after treatment.Improvement more than 0.3 of logMAR value was considered as improved visual acuity.Results There was no significant difference in ABCVA between different treatment groups (F=0.050,P＞0.05).The improved visual acuity in group A and B were great than group C (x2=5.645,6.301;P＜0.05).In group A,B and C,there was no significant difference in ABCVA and improved visual acuity between different OCT patterns (P＞0.05).Improved visual acuity of DRT and CME eyes were higher in group A&B (70.59％ and 50.00％) than in group C (26.47％ and 14.29％),the difference was statistically significant (x2=5.075,4.453;P＜0.05).Conclusions There is no obvious change of visual acuity in patients with different OCT patterns of DME after the same treatment by intravitreal ranibizumab injection and/or laser photocoagulation.The improved visual acuity is not consistent in same OCT patterns after different treatment.
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Objective To investigate the distribution patterns of diabetic macular edema (DME) based on optical coherence tomography (OCT),and explore its correlation with diabetic retinopathy (DR) stages and systemic factors.Methods A total of 135 patients (242 eyes) with type 2 diabetes were included in this retrospective study.There were 75 males (138 eyes) and 60 females (104 eyes),the ages were from 29 to 83 years,with an average age of (5 8.8± 11.1) years.The general information such as height,weight,smoking history and blood glucose [such as glycosylated hemoglobin (HbA1 c)],blood pressure,blood lipid,24 hours urine protein and other examinations were collected.The diagnosis of DR and DME were made,and the staging of DR and typing of DME were performed based on fundus color imaging and OCT.DR were divided into mild nonproliferative DR (NPDR),moderate NPDR,severe NPDR and proliferative DR (PDR).DME were categorizedinto 4 types including sponge-like retinal swelling (SME),cystoid macular edema (CME),serous retinal detachment (SRD) and posterior hyaloid traction (PHT).The correlation between DME types and DR staging were analyzed by x2 test and Fisher exact test.Multivariate logistic regression analysis was used to analyze the correlation between DME types and systemic factors.Results In 242 DR eyes the proportions of mild,moderate,severe NPDR and PDR were 30.99％,32.64％,23.14％ and 13.23％,respectively.There were 199 eyes (82.23％) with DME.There were statistically significant differences in the proportion of DME in different stages of DR (x2=21.077,P＜0.01).In the 199 eyes with DME,There were 165 eyes (68.18％) of SME,22 eyes (9.09％) of CME,7 eyes (2.89％) of SRD and 5 eyes (2.07％) of PHT.The distribution of DME patterns in different stages of DR was statistically significant (x2=156.273,P＜0.01).Logistic regression analysis showed that the duration of diabetes,HbA1c and macroalbummuria were independent risk factors for DME [odds ratio (OR)=1.090,1.510,4.123;P＜0.05],and were also independent for SME (OR=1.092,1.445,3.942;P＜0.05);HbA1c wasanindependentriskfactorforSRD(OR=2.337,P＜0.05).Canclusions There are differences in the distribution of different DME types in each stage of DR.The duration of diabetes,HbA 1c and macroalbuminuria were independent risk factors for DME and SME,and macroalbuminuria and HbA1c for CME and SRD.
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Objective To explore and analyze the effects of allicin prodrug on proliferation of esophageal cancer cell line Eca9706 and the expression of apoptosis gene.Methods Different concentrations of allicin prodrugs were divided into a total of four groups:A1 group(10μg/mL),A2 group(20 μg/mL),A3 group (40 μg/mL),A4 group (normal saline,0 μg/mL),and respectively applicated in esophageal carcinoma cell line Eca9706.After culturing for 1 days,2 days,3 days,cell proliferation was detected by MTT and expression of p53 at the mRNA level was detected by RT-PCR.Results The optimum concentration of proliferation inhibition of allicin prodrug on esophageal cancer cell line Eca9706 was 20μg/mL and the best inhibition time was 2 days;the gene level of esophageal cancer cell line Eca9706 was inhibited with a dose-dependent.Conclusion Allicin prodrugs could effectively inhibit the proliferation of esophageal cancer cell line Eca9706,and control the proliferation of esophageal cancer cells by regulating the expression of apoptosis associated genes,so as to eliminate tumor cells.
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Objective To investigate the efficiency and safety of transfection green fluorescent protein plasmid to mouse cornea mediated by SonoVue and ultrasound.Methods Saline,plasmid+saline,plasmid+SonoVue and liposome+plasmid were injected respectively to mouse eye anterior chamber.Then the mouse eye of plasmid+saline and plasmid+SonoVue injection group were exposed to pulse wave ultrasound under 50 Hz pulse reDetition frequence,2 W/cm2 intensity and 10 minutes duration time.Fluorescence stereomicroscope was used to observe the expression of EGFP in the eye at the 1 st day,3rd day,7th day,14th and 21 st day after injection.Two mice were taken randomly from each group and were sacrificed at the 3rd day after injection.Their eyes were enucleated and made into frozen coronal sections.And fluorescence microscopy was performed to observe the type and distribution of EGFP positive cell.Tissue damage was observed in pathological section.Results EGFP was exDressed over the ocular surface in SonoVue and ultrasound group,and it obviously higher than only ultrasound exDosure group and liposome group.The expression of EGFP was not detect ed over the ocular surface in other groups.Pathological sections were not found any difference in each group.Conclusions SonoVue and ultrasound can successfully and safely transfer gene to ocular in vivo study.
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ObjectiveTo explore the etiology of incision hernia of abdominal wall. Methods 72 cases with incision hernia were analyzed retrospectively in types of incision, technique of suture, materials, infection of incision, increase of intraabdominal pressure, age, nutrition and time of occurrence. ResultsLongitudinal incision, bad technique of suture,infection of incision, increase of intraabdominal pressure, aged, hypoalbuminemia and diabetes mellitus easily induced incision of hernia. ConclusionTransverse incision should be adopted if operation and safety are not affected. Prevent and treat infection or rupture of incision by strict aseptic manipulation, hemostasis and right use of antibiotics. Prevent increase of intraabdominal pressure in every aspect. Pay atention to nutrition supply of the elderly and promote healing of incision. Postoperative half year is high incidence stage of incision hernia and all inducing factors should be avoided.
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0.05). However,life quality was improved and the improvement of clinical symptoms was more obviously in the treatment group as compared with the control group.Conclusion KI combined with chemotherapy and radiotherapy could increase the effects,improve and decrease the side effects.
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Objective To evaluate the polyethersulfone(PES)hollow fiber membrane automatic plasma collector and its efficiency for separating plasma.Methods The plasma was collected by using the automated plasma collector with an innovating PES hollow fiber membrane plasma separator incorporated into a plasmapheresis device,using single needle.The concentration of total plasma protein was determined by Bio Rad method.Results 1)The sieving coefficients were over 95% during the plasma collection, and were stable.2)As the hematocrit (Hct) increased, the plasma collection rate decreased, but the plasma separation efficiency increased.At constant Hct of 0.45,the plasma filtration rate was 23.6ml/min,and the plasma separation efficiency was 52%.It took 20min to collect 200mL plasma.3)The maximum hemolysis rate after several circulation of collection was below 2.5%.Conclusion The plasma collection was satisfactorily performed, and the yield of plasma protein components was nearly the same as the plasma in whole blood.The PES membrane collector had a comparatively high efficiency of plasma separation.